The software contains multiple plate displays, which are recognizable by their resemblance of the arrangement of wells in the instrument itself. Depending on what analysis module you are using, the Sample as Plate display will represent different information about the wells. Please open the “MyGo Pro 2 fold Dilution series.ppf” file.
Sample Plate Displays
When in Samples the Samples as Plate will look as follows:
Each entire circle represents a well in the instrument. The position of the well in the instrument is consistent with its position in the plate display. This plate display shows the arrangement of 32 wells in an array of 8 strips of 4, in a MyGo Pro instrument.
Sample Plate Colours
The colour in the centre of the circle represents the sample colour. The outer ring colour represents the target assigned to that sample. The type of target is indicated by the small icon situated to the left of the inner circle boundary. The black and white dotted line around the well is shows the user which well is being selected in the table.
Sample Plate Targets
There are 3 types of targets that can be assigned to a particular sample. In the example below the sample has a target assigned to it that is used as a standard in the experiment. This is indicated by the yellow positive symbol.
In the next example the sample has a target assigned to it that has been used as a negative during the experiment. This is represented by a blue negative symbol.
In the final example neither a standard or a negative has been assigned to it. This is represented with the absence of symbols.
Sample Plate Legends
Below is the legend that you will see in the Samples pane that will help you in using the Samples as Plate view. The colours in the legend depending on what sample and target colours you have assigned to the first sample in the samples table.
Quantification Plate Displays
When using Auto. Quant. or Fit Point Quant. the Samples as Plate will look as follows:
Again, the position of the well in the instrument is consistent with its position in the plate display. The inner circle represents the sample colour like in the Samples display. However, unlike the Samples display, the outer circle indicates concentration which is represented by the radius of the outer ring. The radius is inversely proportional to the Cq, therefore the larger the radius the lower the Cq value for that sample, thus the higher the starting concentration of template used for that sample.
Below is the legend that you will see in the quantification Samples as Plate pane that will help you in using the Samples as Plate view. Quantification modules can also be used for more challenging applications like 6 plex multiplexing. Please open the “MyGo Pro P plex.ppf” file from the demo data folder. Select Auto. Quant. and then Samples as Plate. Select one of the wells from the bottom row and the legend should now look like the figure below.
In this example the sample, indicated by the inner circle is blue. When more than one target has been assigned to the sample the outer radius becomes partitioned depending on the amount of targets. In this example the sample has 6 different targets assigned to it. Each of these targets are coming up at different Cq values, represented by differently sized outer segments.
Other Analysis Plate Displays
Samples as Plate for other analyses such as TM Calling, Spectra View and Endpoint Genotyping is as follows:
As before, the well position in the instrument corresponds to the position in the mount. The only other indicator needed for this display is the sample colour. In this case all of the samples in this plate display are blue.