Introduction

Each experiment requires Run Settings. This tells the software what is present in your PCR reaction.

Run Settings

Run Settings is the 2nd tab on the left hand side of the screen and when selected shows the following display. This display is annotated to help cover the available options.

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Optics Settings

There are 3 main choices that will affect how an experiment runs when not using Advanced Settings (8). These are: dye present (1-3), signal quality (4-6) and the option to turn on optimize Melt Acquisitions (7).

Intercalating Dyes (1)

This should be used if the experiment contains an intercalating dye, for example SYBR Green.

Hydrolysis Probes (2)

This should be used if the experiment contains hydrolysis probes, for example FAM, HEX.

Other Dyes (3)

This should be used if the experiment contains other dyes, for example, Molecular Beacons.

High Quality (4)

This will improve data quality by averaging over an increased amount of data points.

Normal Quality (5)

This will use the default number of data points and should be used if you are unsure.

High Speed (6)

This uses the minimum amount of data points to reduce the time taken for optical acquisitions. This can be used for a high speed reaction.

Optimize Melt Acquisitions (7)

This option tells the MyGo Pro to find the best settings to use during melting. This can also be used to optimize integration times during cycling. This will be covered in Optimize Integration Times.

Use Advanced Settings (8)

Advanced settings allow the user to specify in detail how optical integrations are performed. This will be covered in more detail in Optimize Integration Times, further on in the manual.

Display Dye Calibration (9)

This shows the dye calibrations that are stored in the current experiment, and which are used to analyse optical data. This setting is not required to run an experiment and will be covered in more detail in Automatic Dye File Generation.

Fused Project Run Settings

When projects are fused together the run settings looks different. Below Experiment 1 and 2 have been fused together.

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As you can see we are given an settings summary of the run settings that were used in experiment 1 and 2.