This section will teach you everything you need to know to get started with MyGo Mini experiments. You will learn how to create, save, open and close experiments. You will learn how to set up an experiment including profile, sample setup and run settings. Finally we will show you how to run your new experiment from the software and from the USB provided.
Create a new experiment
Below is the MyGo mini home screen. By selecting New you will create a new experiment.
Once you have created a new experiment the Experiment Summary will be displayed as shown below:
By default the experiment name will be called New Experiment with a date and time stamp, which can be edited. The summary will give you information about the instrument you are running, the experiment and give you the option to add any useful notes about your experiment.
Open an experiment
By selecting Open you can open an experiment as shown below:
Files as Type:
The Files as Type drop down menu will give you the option to open the experiment as an experiment file or as a template file.
An experiment file will contain all the data and analyses but can not be re run. To open as an Experiment File select the option shown below:
Experiment File as Template
A template file will contain all previous protocols from the experiment you are opening that can be run again. It will also contain all previous sample setups and analyses that do not need to be re-added. If you wish to open an Experiment File as Template select the option shown below:
Saving an experiment
Once an experiment has completed it can either be automatically saved to a location on your computer or manually saved at a time of your choosing. The experiment must be saved before the software is closed to ensure the data is not lost.
When starting a run you will be prompted with the following window:
To automatically save your data when the experiment has finished, select the first option. To choose the location to save your run to select Browse… and you will be prompted to choose a file directory. To prevent the software from showing this dialog box, select the second option.
You can still change these settings by selecting Configuration and selecting General Config. as shown below:
Once your experiment has finished you can save your experiment manually by selecting Save or Save as. If your experiment is unsaved, you will be prompted to choose a location to save your experiment. If already saved you can save any changes made to the experiment by selecting Save, which will overwrite the previous version.
Closing an experiment
To close an experiment select Close. If the experiment has not been saved the following dialog box will appear:
If you do not wish to close, select Do not close. If you would like to save your data select Save and close. If you do not wish to save the data select Close, losing data. The following dialog box will appear if you choose to close without saving:
The option to close the data will be available after waiting 3 seconds. You still have the option to not close the data and return to the experiment by selecting Do not close.
Setting up a profile
A profile is required to perform an experiment. After creating a new experiment select the Profile tab as shown below:
To add a program to your experiment select Add in the Programs window. Programs can be deleted by selecting Delete. the program order can also be altered by selecting the Up and Down arrows.
Once programs have been added to the experiment they will appear in the Temperature Profile window in the order they are present in the Programs window as shown below:
To alter the settings of a particular program you should first select it, then refer to the right hand window, in this case the Melting window. In the example above the Melting program is selected and can be edited in the right hand window.
You will now learn how to set up samples on you MyGo Mini. Samples or targets do not need to be added to the experiment for an experiment run. They can be added to an experiment that has already completed. With a new experiment created please select the Samples tab as shown below:
Samples can be added and removed from the experiment by selecting + and - in the Samples window. The order of samples in the Samples window can be altered by selecting the Up and Down arrows in the Samples window.
Assigning samples to wells
After adding samples to the experiment they can be assigned to wells by first selecting the wells you wish to assign them to, and then select Set. In the example below Sample 1 has been assigned to wells 1, 2, 3 and 4. Samples can also be unassigned from wells by selecting the wells you wish to un-assign and select Clear.
Targets can be added and removed from the experiment by selecting + in the Targets window. The order of targets in the target window can be altered by selecting the Up and Down arrows in the Target window. In the example below a target has been added to the Target Window:
Changing and assigning targets
The dye that is used for a specific can be changed by selecting the Dye drop down menu in the Targets window. In the example below, SYBR has been changed to FAM. This target can then be assigned to specific wells as a Standard, Unknown or Negative by selecting Std., Unk., and Neg., respectively. Targets assignments can be cleared by selecting Clear with the target and wells you wish to clear. In the example below, FAM has been assigned to wells 1, 2, 3 and 4 as Unknown:
Saving and opening a sample setup
If you wish to save a sample setup you can select Save As., from the bottom right corner of the display above. These can be saved as a csv. and psd. files.
csv. sample file
This file can be opened and edited by a text editor or a spreadsheet program.
psd. sample file
This can not be opened or edited once saved.
Sample setups can be opened by selecting Open.
The MyGo Mini software provides default run settings that don’t need to be edited if the user does not wish. To view them, select Run Setting which are shown below:
The defaults optics settings are as follows:
|Cycling Acquisitions||Integration Time (s)||4|
|Acquisitions per Cycle||5|
|Melting Acquisitions||Integration Time for Melt (s)||0.5|
The settings have been optimised for many different PCR reagents and do not need to be changed. However, if you wish to change them you do have that option and can do so by entering your desired settings into the number fields.
Display Dye Calibrations
By selecting Display Dye Calibrations from the bottom left of the Optics Settings window you will be shown the dye calibrations as shown below:
The Dye Calibrations will appear on the right of the screen. Only when you have connected to an instrument or opened an experiment will the dye calibrations appear. Dyes can be opened and used in an experiment, saved from an instrument or update from a device.
Starting a run from the software
To start a run from the MyGo Mini software select Start Run. You will then be presented with the auto save options (unless you have chosen not to be prompted) and then be asked to chose an instrument from the list of Registered Instruments. Select an instrument and press Select to begin the run.
Starting a run from a USB stick
To start a run, repeat Starting a run from the software, however when you are asked to choose an instrument, select Start from USB. You will then be prompted to find the location of your USB. Once selected press Save and safely remove your USB. Your USB can now be placed into the instrument and your experiment will start automatically.