The MyGo system they displays different coloured lights when running to indicate what the instrument is doing at that given time. This section will help you to learn what these different colours mean. This section will also teach you more about the functionality of the heated lid for both systems, including temperatures, preheating and standby modes.
MyGo Pro Display Colours
The MyGo Pro communicates silently across the lab by displaying different coloured lights as follows:
When you power on your MyGo Pro the instrument will begin to initialise. During initialisation the instrument will display blue (whether the lid is open or closed).
Ready to run / Standby
Once the instrument has completed initialising it will display red if the lid is open and will display green if the lid is closed. We encourage users to keep the lid closed whenever possible as the lid may be hot and keeping the lid closed will also prevent dust from falling into the wells.
At this point the MyGo Pro is ready to for loading tubes into. he lid will not lock until tubes have been loaded.
Once tubes have been loaded, the lid can be closed, and will lock automatically, the instrument will then display yellow.
Whilst performing PCR the MyGo Pro will go through several cycling stages each indicated by various colours as described below:
When the run has started the instrument will go blue which means the heated lid is first heating up to 105ºC. Once the heated lid has met this temperature, thermal cycling will begin. The heated lid will remain at 105ºC during thermal cycling.
When the instrument is at 95oC the instrument will display red.
When the instrument is at 50oC it will go back to displaying blue.
At temperatures between 60oC and 95oC the instrument will slowly change the display from blue to red, via pink.
Acquiring Optical Data
When the instrument is acquiring data it will flash blue. Note that during a melt, optical data is acquired continuously, and so the display will flash blue throughout the melt.
When the instrument has finished its experiment, the lid will open and the light will go cyan. To prepare for another run, open the lid, remove the tubes and close the lid again.
Once the tubes have been removed from the instrument it will go into standby mode, and once more display green when the lid is closed.
MyGo Mini Displays
The MyGo Mini uses a combination of sounds and displays to indicate what it is doing. Here is a description of each of the stages.
When the MyGo mini powers on it will first pulse blue for a few seconds and then go blank before going green. This indicates that the instrument has completed its power on self test.
Ready to run / Standby
The MyGo mini will finish its power on self test by making a sound and going green. This is the MyGo Mini’s standby mode.
When you fit the lid the instrument will say “lid on.” During standby mode the MyGo Mini will remain green if the lid is on.
When you remove the lid in standby mode the instrument will say “lid off” pulse yellow. When not using the instrument, please leave the lid fitted to prevent dust from falling into the wells.
Like the MyGo Pro the MyGo Mini will display different colours to indicate different stages of the thermal cycling profile. These stages are the same as the MyGo Pro displays.
Once the run is complete the instrument will say “analysis complete” and will go multicoloured (a slowly moving rainbow display). To prepare for another run, remove the lid, remove the tubes and then fit the lid again.
Once the tubes have been removed the instrument will go into standby mode.
The lids for the MyGo instruments work slightly different from one another.
When the MyGo instruments are turned on the heated lids will be in standby mode which means it will be turned off in order to save energy.
If the user opens the lid and closes it during standby mode the heated lid will preheat to 105ºC for 5 minutes. If the instrument remains inactive after 5 minutes the lid will turn off. The same behaviour is experienced if the lid is opened and then locked. Even when locked the lid will turn off after 5 minutes of inactivity. The MyGo Mini does not have a preheating functionality.
During cycling the heated lids for the MyGo Pro and the MyGo Mini will remain at 100ºC or above.
When an experiment is complete the MyGo Pro heated lid will remain hot for 5 minutes. This is in case the user wants to run an additional experiment without waiting for the heated lid to reach its desired temperature. After 5 minutes of inactivity the heated lid will cool down and return to standby. The MyGo Mini heated lid will return to standby mode as soon as the experiment has been completed. Please be aware that when you remove the MyGo Mini lid immediately after completing a run, the heated lid inside may be hot - please do not touch these parts. The top surface of the lid should be used to grip the lid - note that it may become warm during a run, this is normal.